Double immunohistochemistry to detect transglutaminase 2-IgA deposits in celiac children: a multicentre study.

Cohort studyDigestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver· Jul 2026

Double immunohistochemistry to detect transglutaminase 2-IgA deposits in celiac children: a multicentre study.

Carla Giordano, Monica Montuori, Paola Parente … Salvatore Oliva

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Gastrointestinal & Liver PathologyCohort studyQ1IF 3.2

Double immunohistochemistry detected TGM2-IgA deposits in all celiac disease biopsies with villous atrophy

Double immunohistochemistry to detect transglutaminase 2-IgA deposits in celiac children: a multicentre study.

Carla Giordano … Salvatore Oliva

Digestive and liver disease · Jul 2026

Background

Intestinal transglutaminase 2 (TGM2)-IgA deposits represent an early marker of celiac disease (celiac disease).

Purpose

This multicentre retrospective study aimed to assess the usefulness of a double immunohistochemistry technique for detecting TGM2-IgA deposits in formalin-fixed, paraffin-embedded duodenal biopsies from patients with celiac disease.

Methods

A small cohort of celiac disease patients was analysed both at diagnosis and after a gluten-free diet.

n = 80 control

Results

TGM2-IgA deposits were detected in every biopsy showing villous atrophy

CeD

100%

pCeD (Marsh 0/1)

72%

Controls

0%

More results

Among 17 celiac disease patients re-evaluated after a gluten-free diet, all achieved mucosal healing (Marsh 0), 65% showed complete disappearance of deposits, and the remainder a marked reduction.

“

Conclusion

TGM2-IgA double immunohistochemistry may help to refine diagnostic algorithms for celiac disease diagnosis.

Double immunohistochemistry to detect transglutaminase 2-IgA deposits in celiac children: a multicentre study.

Double immunohistochemistry to detect transglutaminase 2-IgA deposits in celiac children: a multicentre study.

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