Possible involvement of NETosis in inflammatory processes in the eye: Evidence from a small cohort of patients.
More complicated proliferative diabetic retinopathy had higher vitreous myeloperoxidase levels.
Possible involvement of NETosis in inflammatory processes in the eye: Evidence from a small cohort of patients.
To evaluate whether NETosis is involved in cytokine-induced ocular inflammation and to track neutrophil extracellular traps (neutrophil extracellular traps) complexes in patients with proliferative diabetic retinopathy (proliferative diabetic retinopathy).
For the animal model, the eyes of C57BL/6J mice were intravitreally injected with interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-α), or saline.
Vitreous myeloperoxidase levels were higher in patients with more complicated proliferative diabetic retinopathy.
Massive influx of CD11+ inflammatory cells, involving the anterior and posterior chambers, was observed in the murine eyes 24 h after the IL-8 or TNF-α injections.
Cells excreted to their surroundings an extracellular net-like structure positive for neutrophil elastase, myeloperoxidase, and H3Cit.
H3Cit staining was abolished with the DNase I treatment, indicating the presence of extracellular DNA in the net-like structures.
This study showed the existence of NETosis in cytokine-induced ocular inflammation in a mouse model and human samples.
Furthermore, the extent of neutrophil extracellular traps complex formation was higher in a subset of patients who exhibited more complicated proliferative diabetic retinopathy.