Efficacy and Limitations of Flow Cytometry for the Rapid Diagnosis of Primary Central Nervous System Lymphoma.
Flow cytometry detected primary CNS lymphoma with 85.7% sensitivity, 100% specificity
Efficacy and Limitations of Flow Cytometry for the Rapid Diagnosis of Primary Central Nervous System Lymphoma.
Primary central nervous system lymphoma (primary central nervous system lymphoma) has a markedly high proliferation rate, making early diagnosis and prompt therapeutic intervention essential.
We retrospectively analyzed 67 consecutive patients with suspected primary central nervous system lymphoma who underwent intraoperative flow cytometry between 2010 and 2023 based on preoperative imaging.
flow cytometry correctly identified lymphoma in most true cases, missing some
Using histopathology, we confirmed the presence of primary central nervous system lymphoma in 42 patients, all diagnosed as having diffuse large B-cell lymphoma.
Six cases (14.3%) were discordant (FCM-D).
T-cell markers were significantly elevated in FCM-D cases ( p < 0.01), although these were pathologically diagnosed as diffuse large B-cell lymphoma based on histology and immunohistochemistry.
Flow cytometry yields reliable diagnostic information within hours of tissue collection and supports early therapeutic decisions in primary central nervous system lymphoma. Discordant results may reflect reactive T-cell infiltration.
This is the first study to present detailed subset analyses in primary central nervous system lymphoma using flow cytometry in correlation with pathology, underscoring its utility as a rapid diagnostic tool.