Intraoperative Molecular Profiling of Sentinel Lymph Nodes: Diagnostic Value of Mammaglobin and CK19 in Early Breast Cancer.

This descriptive study included 301 sentinel lymph node biopsies from patients with stage I-II breast cancer treated at the San Carlos Clinical Hospital in Madrid, Spain.

Metastases were identified using conventional histopathology (H&E), immunohistochemistry (immunohistochemistry), and molecular detection of mammaglobin and CK19 using PCR-based methods.

The overall sensitivity was 68.9%, and the specificity was 93.42%.

Mammaglobin showed slightly better diagnostic performance than CK19, and the combined molecular detection of both genes improved diagnostic accuracy when compared with individual markers.