Validation of NTRK Fusion Detection Using an Ultrarapid, Fully Automated Cartridge-Based PCR Assay.

Of 108 NTRK gene fusions detected by next-generation sequencing, 67 (62.0%) were reported as detected by Idylla.

Including equivocal results as positive increased sensitivity (positive percentage agreement, 96.3%) with a modest specificity reduction (negative percentage agreement, 93.9%; n = 190).

Most invalid results reflected RNA degradation and resolved with freshly cut sections.