A Novel Anti-CDH5/VE-Cadherin Monoclonal Antibody (Ca 5 Mab-8) for Flow Cytometry, Western Blotting, and… | ScholarDeck

Paper

A Novel Anti-CDH5/VE-Cadherin Monoclonal Antibody (Ca 5 Mab-8) for Flow Cytometry, Western Blotting, and Immunohistochemistry.

New research · Pathology

Monoclonal antibodies in immunodiagnosis and immunotherapy · 3w

Animal / preclinicalMonoclonal antibodies in immunodiagnosis and immunotherapy· 2026

A Novel Anti-CDH5/VE-Cadherin Monoclonal Antibody (Ca 5 Mab-8) for Flow Cytometry, Western Blotting, and Immunohistochemistry.

Haruto Yamamoto, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

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HematopathologyAnimal / preclinicalQ4IF 0.8

New monoclonal antibody Ca 5 Mab-8 binds CDH5 with high affinity, constant 6.1 × 10⁻⁹ M

A Novel Anti-CDH5/VE-Cadherin Monoclonal Antibody (Ca 5 Mab-8) for Flow Cytometry, Western Blotting, and Immunohistochemistry.

Haruto Yamamoto … Yukinari Kato

Monoclonal antibodies in immunodiagnosis and immunotherapy · 2026

Background

Cadherin-5 (CDH5), also known as vascular endothelial cadherin (VE-cadherin), plays crucial roles in endothelial cell adhesion, vascular barrier function, and signaling.

Results

6.1 × 10⁻⁹ M

antibody grips CDH5 tightly, indicating strong, stable target binding

More results

Here, new anti-human CDH5 mAbs, called Ca 5 Mabs, were developed through a flow cytometry-based high-throughput screening.

Among them, clone Ca 5 Mab-8 (mouse IgG 2a , kappa) recognized CDH5-overexpressed Chinese hamster ovary-K1 (CHO/CDH5) cells in flow cytometry.

These reactivities exceeded those of a commercial anti-CDH5 mAb (clone BV9).

More results

Ca 5 Mab-8 can detect endogenous CDH5 in Western blotting.

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Conclusion

These findings suggest that Ca 5 Mab-8 is a versatile basic research tool in vascular biology and has potential for clinical applications including tumor diagnosis and therapy.

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