Diagnostic Concordance and Superiority of Flow Cytometry Over Immunohistochemistry in Acute Leukemia…

Cohort studyMedical science monitor : international medical journal of experimental and clinical research· Aug 2026

Diagnostic Concordance and Superiority of Flow Cytometry Over Immunohistochemistry in Acute Leukemia Immunophenotyping: A Single-Center Study.

Gülçin Dağlıoğlu, Tuğba Toyran, Özlem Görüroğlu Öztürk … Tamer Cevat İnal

HematopathologyCohort studyDiagnosisQ2IF 2.4

Flow cytometry showed only 61% concordance with immunohistochemistry for B-cell acute lymphoblastic leukemia

Diagnostic Concordance and Superiority of Flow Cytometry Over Immunohistochemistry in Acute Leukemia Immunophenotyping: A Single-Center Study.

Gülçin Dağlıoğlu … Tamer Cevat İnal

Medical science monitor · Aug 2026 · Q2 in General Medicine

Background

Early diagnosis is crucial for effective treatment and management of acute leukemia.

Purpose

This study aimed to compare the diagnostic agreement between flow cytometry and histopathological evaluation using shared parameters from bone marrow samples.

Methods

MATERIAL AND METHODS A total of 144 patients presenting to Adult Hematology, Pediatric Hematology, and Pediatric Oncology clinics at Çukurova University Faculty of Medicine were enrolled; bone marrow samples were processed at the Central Laboratory flow cytometry Unit.

n = 144 patients

Results

immunohistochemistry backup confirms B-ALL calls only about 3 in 5 times

AML

82.6%

B-ALL

61%

More results

Flow cytometry achieved 95% sensitivity for AML and 100% for B-ALL.

CD33, MPO, and CD117 showed significant concordance in AML, with sensitivities of 94%, 91%, and 90%, respectively.

CD34 was the top-performing biomarker for AML, with 93% sensitivity and 95% specificity.

More results

For B-ALL, TdT, CD33, and CD34 showed sensitivities of 77%, 75%, and 81%, respectively.

“

Conclusion

To quantitatively analyze marker expression with high sensitivity, precise lineage differentiation, and a quick turnaround time, same-day reporting and rapid initiation of targeted therapy make flow cytometry essential in modern hematopathology.

Diagnostic Concordance and Superiority of Flow Cytometry Over Immunohistochemistry in Acute Leukemia Immunophenotyping: A Single-Center Study.

Diagnostic Concordance and Superiority of Flow Cytometry Over Immunohistochemistry in Acute Leukemia Immunophenotyping: A Single-Center Study.

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