Impact of DNA Methylation Profiling on Central Nervous System Tumor Diagnosis and Management: A Pediatric…

Cohort studyTurkish neurosurgery· 2026

Impact of DNA Methylation Profiling on Central Nervous System Tumor Diagnosis and Management: A Pediatric Cohort Study from Türkiye.

Mehmet Arda Inan, Ayca Ersen Danyeli, Figen Soylemezoglu … Memet Ozek

Molecular PathologyCohort studyDiagnosisQ3IF 0.9

Methylation profiling matched histopathology in two-thirds of evaluable pediatric central nervous system tumors.

Impact of DNA Methylation Profiling on Central Nervous System Tumor Diagnosis and Management: A Pediatric Cohort Study from Türkiye.

Mehmet Arda Inan … Memet Ozek

Turkish neurosurgery · 2026

Purpose

To present a single-center experience, and to highlight the urgent need for multi-institutional collaboration in Türkiye and surrounding regions lacking access to methylation platforms, with the aim of enhancing diagnostic precision and neuropathological practice.

Methods

All pediatric patients who underwent methylation?based tumor classification (MBTC) between November 2023 and July 2025 were retrospectively identified.

n = 11 cases

Results

methylation matched the biopsy diagnosis in about two-thirds of classifiable tumors

Overall cohort concord

58%

Evaluable tumors conco

67%

More results

A total of 48 tumors were profiled (26 females [54%]; 22 males [46%]; median age, 6.5 years; range, 0?17).

The most frequent localization was supratentorial (n=18, 36%).

Of the entire cohort, concordance was 58%.

Discordance occurred in 11 cases (23%), including 6 (13%) with major discrepancies.

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Conclusion · 1 of 2

MBTC is a valuable complementary tool in the diagnostic workup of pediatric central nervous system (central nervous system) tumors, particularly in morphologically ambiguous and ultra-rare cases.

Conclusion · 2 of 2

It should be integrated with conventional histopathology rather than viewed as a replacement, as it may prevent prognostic misclassification and inappropriate treatment in selected patients.

Impact of DNA Methylation Profiling on Central Nervous System Tumor Diagnosis and Management: A Pediatric Cohort Study from Türkiye.

Impact of DNA Methylation Profiling on Central Nervous System Tumor Diagnosis and Management: A Pediatric Cohort Study from Türkiye.

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