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New research · Ophthalmology
PloS one · 15h
Animal / preclinicalPloS one · 2026

Transscleral photodynamic therapy with a chlorin e6: An experimental study of exposure parameters and therapeutic window.

Ernest V Boiko, Elena V Samkovich, Irina E Panova … Alexandra E Kim
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OphthalmologyAnimal / preclinical

In rabbits, temperature rise up to 4.5°C gave selective choroidal damage without retinal or scleral injury.

Transscleral photodynamic therapy with a chlorin e6: An experimental study of exposure parameters and therapeutic window.

Ernest V Boiko … Alexandra E Kim
PloS one · 2026
Purpose

To define optimal exposure parameters and the therapeutic window for transscleral photodynamic therapy (transscleral photodynamic therapy) with chlorin e6 by evaluating clinical, histological, and thermal effects of subthreshold, therapeutic, and suprathreshold settings in rabbit eyes.

Methods

The study was conducted on 21 healthy rabbits.

n = 21 healthy rabbits
ΔT ≤ 4.5°C
Results
ΔT ≤ 4.5°C
Keeping tissue heating under 4.5°C spared the retina and sclera during treatment.
n = 21 healthy rabbits
More results

Fundus examination on day 14 revealed distinct treatment zones correlating with laser settings.

Suprathreshold settings (≥0.3 W for 5 mm; ≥ 0.6 W for 10 mm) induced retinal necrosis (up to 50%) and scleral coagulation (ΔT ≥ 8°C) with power densities exceeding 0.866 W/cm² (5 mm) and 0.764 W/cm² (10 mm).

“
Conclusion · 1 of 2

Transscleral photodynamic therapy with chlorin e6 enables selective targeting of intraocular pathological tissues while preserving scleral and retinal integrity.

Conclusion · 2 of 2

Defining the therapeutic window and using real-time thermal monitoring enhances treatment safety. These findings lay a foundation for clinical protocols for uveal melanoma and other intraocular tumors.

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