Transscleral photodynamic therapy with a chlorin e6: An experimental study of exposure parameters and…

Animal / preclinicalPloS one· 2026

Transscleral photodynamic therapy with a chlorin e6: An experimental study of exposure parameters and therapeutic window.

Ernest V Boiko, Elena V Samkovich, Irina E Panova … Alexandra E Kim

Ocular OncologyAnimal / preclinicalQ1IF 3.0

In rabbits, temperature rise up to 4.5°C gave selective choroidal damage without retinal or scleral injury.

Transscleral photodynamic therapy with a chlorin e6: An experimental study of exposure parameters and therapeutic window.

Ernest V Boiko … Alexandra E Kim

PloS one · 2026

Purpose

To define optimal exposure parameters and the therapeutic window for transscleral photodynamic therapy (transscleral photodynamic therapy) with chlorin e6 by evaluating clinical, histological, and thermal effects of subthreshold, therapeutic, and suprathreshold settings in rabbit eyes.

Methods

The study was conducted on 21 healthy rabbits.

n = 21 healthy rabbits

ΔT ≤ 4.5°C

Results

ΔT ≤ 4.5°C

Keeping tissue heating under 4.5°C spared the retina and sclera during treatment.

n = 21 healthy rabbits

More results

Fundus examination on day 14 revealed distinct treatment zones correlating with laser settings.

Suprathreshold settings (≥0.3 W for 5 mm; ≥ 0.6 W for 10 mm) induced retinal necrosis (up to 50%) and scleral coagulation (ΔT ≥ 8°C) with power densities exceeding 0.866 W/cm² (5 mm) and 0.764 W/cm² (10 mm).

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Conclusion · 1 of 2

Transscleral photodynamic therapy with chlorin e6 enables selective targeting of intraocular pathological tissues while preserving scleral and retinal integrity.

Conclusion · 2 of 2

Defining the therapeutic window and using real-time thermal monitoring enhances treatment safety. These findings lay a foundation for clinical protocols for uveal melanoma and other intraocular tumors.

Transscleral photodynamic therapy with a chlorin e6: An experimental study of exposure parameters and therapeutic window.

Transscleral photodynamic therapy with a chlorin e6: An experimental study of exposure parameters and therapeutic window.

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