Performance of the ISOLD Score (Interleukin Score for IntraOcular Lymphoma Diagnosis) in the Diagnosis of…

StudyAmerican journal of ophthalmology· Jul 2026

Performance of the ISOLD Score (Interleukin Score for IntraOcular Lymphoma Diagnosis) in the Diagnosis of Vitreoretinal Lymphoma in Clinical Practice.

Martin Chevalier, Elise Sourdeau, Magali Le Garff-Tavernier … Bahram Bodaghi

Ocular OncologyStudyDiagnosisQ1IF 4.2

ISOLD score accurately diagnosed vitreoretinal lymphoma from aqueous humor samples.

Performance of the ISOLD Score (Interleukin Score for IntraOcular Lymphoma Diagnosis) in the Diagnosis of Vitreoretinal Lymphoma in Clinical Practice.

Martin Chevalier … Bahram Bodaghi

American journal of ophthalmology · Jul 2026 · Q1 in Ophthalmology

Purpose

To evaluate the real-world diagnostic performance of the ISOLD score-a probabilistic formula based on absolute intraocular IL-10 and IL-6 levels-and to compare it with absolute cytokine thresholds and the IL-10/IL-6 ratio for vitreoretinal lymphoma (vitreoretinal lymphoma) diagnosis.

Methods

One hundred sixty-six patients who underwent intraocular sampling for IL-6 and IL-10 measurement at a tertiary vitreoretinal lymphoma referral center between January 2017 and June 2019, yielding 183 samples (156 aqueous humor from anterior chamber paracentesis and 27 vitreous).

n = 183 samples

Results

test correctly told apart lymphoma from non-lymphoma eye-fluid samples nearly every time

86.8%

Sensitivity

98.6%

Specificity

More results

At a threshold of 0, the ISOLD score yielded overall sensitivity of 86.8%, specificity of 98.6%, PPV of 94.3%, and NPV of 96.6% across all 183 samples.

More results

The IL-10/IL-6 ratio at a threshold of 0.6 in aqueous humor (n=150) demonstrated sensitivity 77.8%, specificity 93.9%, PPV 63.6% and NPV 96.9%; at a threshold of 1 in vitreous (n=27), sensitivity, specificity, PPV, and NPV were 80%, 100%, 100%, and 63.6%, respectively.

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Conclusion · 1 of 3

The ISOLD score is a reliable tool for vitreoretinal lymphoma diagnosis in routine clinical practice, with performance validated primarily on aqueous humor samples obtained by anterior chamber paracentesis.

Conclusion · 2 of 3

It outperforms the IL-10/IL-6 ratio alone by providing a continuous probabilistic estimate. False-negative results may occur in immunosuppressed patients or those receiving corticosteroids or chemotherapy.

Conclusion · 3 of 3

A larger vitreous sample would be required to draw definitive conclusions regarding ISOLD performance on this specimen type.

Performance of the ISOLD Score (Interleukin Score for IntraOcular Lymphoma Diagnosis) in the Diagnosis of Vitreoretinal Lymphoma in Clinical Practice.

Performance of the ISOLD Score (Interleukin Score for IntraOcular Lymphoma Diagnosis) in the Diagnosis of Vitreoretinal Lymphoma in Clinical Practice.

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