Immunohistochemistry-Based Screening for Targetable Kinase Alterations in Non-Langerhans Cell Histiocytosis with Juvenile Xanthogranuloma morphology.

All immunohistochemistry-positive cases were confirmed by FISH.

Targeted next-generation sequencing identified fusion genes in analyzable cases, including KIF5B::ALK and TPM3::NTRK1, with RNA-based sequencing being limited by specimen quality in some cases, while DNA-based comprehensive genomic profiling showed higher feasibility in recently processed samples.