Clinical application of chest CT image screening combined with nanopore targeted sequencing in improving the diagnosis rate of pulmonary tuberculosis patients.

Radiologists' experience in pulmonary tuberculosis imaging diagnosis can reduce missed diagnoses.

Additionally, combining nanopore targeted sequencing with conventional microbiological tests helps improve early diagnosis in suspected pulmonary tuberculosis patients.

The combined use of nanopore targeted sequencing and Xpert MTB/RIF yielded the highest diagnostic accuracy (AUC 0.990).