Surgical Murine Model of Congenital Diaphragmatic Hernia.

Animal / preclinicalThe Journal of surgical research· Aug 2026

Surgical Murine Model of Congenital Diaphragmatic Hernia.

Jessica G Wright, Sophia G Hameedi, Meghan E Hovell … Oluyinka O Olutoye

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Hernia & Abdominal WallAnimal / preclinicalQ2IF 1.6

Surgically created congenital diaphragmatic hernia lowered fetal lung-to-body-weight ratio versus sham and controls.

Surgical Murine Model of Congenital Diaphragmatic Hernia.

Jessica G Wright … Oluyinka O Olutoye

The Journal of surgical research · Aug 2026 · Q2 in Surgery

Background

Mouse models of congenital diaphragmatic hernia (congenital diaphragmatic hernia) are confounded by the teratogenic effects of nitrofen.

Methods

The fetuses of E16.5 pregnant C57BL/6 mice underwent thoracotomy and creation of a defect in the left hemidiaphragm.

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Results

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Congenital diaphragmatic hernia lungs weighed less relative to body weight than sham or control fetuses

More results

Overall experimental survival was 58% with a successful congenital diaphragmatic hernia creation rate of 19%.

Fetuses with congenital diaphragmatic hernia also had lower airspace percentage (32.78 ± 17.1%) on histologic evaluation when compared to both sham (50.12 ± 10.2%, P = 0.046) and control (55.52 ± 1.4%, P = 0.009) groups.

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Conclusion

A surgical murine model of congenital diaphragmatic hernia is feasible and replicates the characteristic pulmonary findings of congenital diaphragmatic hernia without the confounding teratogenic effects of nitrofen.

Surgical Murine Model of Congenital Diaphragmatic Hernia.

Surgical Murine Model of Congenital Diaphragmatic Hernia.

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