Validation of parathyroid hormone measurement in fine-needle aspiration biopsy washings according to the…

Cohort studyClinica chimica acta; international journal of clinical chemistry· Apr 2026

Validation of parathyroid hormone measurement in fine-needle aspiration biopsy washings according to the IFCC LEAP checklist.

Ewa Kozłowska, Witold Chudziński, Aleksandra Kumorek, Olga Ciepiela

CytopathologyCohort studyDiagnosisQ1IF 4.1

Parathyroid hormone levels in needle biopsy washings agreed with histopathology in 89% of cases.

Validation of parathyroid hormone measurement in fine-needle aspiration biopsy washings according to the IFCC LEAP checklist.

Ewa Kozłowska … Olga Ciepiela

Clinica chimica acta; international journal of clinical chemistry · Apr 2026 · Q1 in General Medicine

Background

Of parathyroid hormone in fine-needle aspiration biopsy washings (FNAB-PTH) is widely used in clinical practice to confirm parathyroid tissue origin and support lesion localization, particularly when imaging findings are inconclusive.

Methods

FNAB washings were analyzed using the Elecsys PTH assay on the cobas platform.

n = 100 cases

89.0%

Results

89.0%

PTH results matched biopsy diagnosis in most cases

n = 100 cases

More results

FNAB-PTH demonstrated excellent precision (CV ≤10%), preserved linearity, negligible carryover, and acceptable analytical sensitivity.

More results

A pronounced concentration-dependent matrix effect was observed in saline washings, with median recovery increasing from 46.9% at low to 76.5% at high PTH concentrations; this effect was markedly reduced after protein supplementation.

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Conclusion

Analytical validation of FNAB-PTH according to the IFCC Laboratory Evaluation and Analytical Performance framework confirms good analytical quality and supports its clinical utility for confirming parathyroid tissue origin and assisting lesion localization, despite the presence of a concentration-dependent matrix effect.

Validation of parathyroid hormone measurement in fine-needle aspiration biopsy washings according to the IFCC LEAP checklist.

Validation of parathyroid hormone measurement in fine-needle aspiration biopsy washings according to the IFCC LEAP checklist.

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