Effect of database, pre-processing pipeline, and scan duration on rs-fMRI functional connectivity based treatment targets for TMS obtained using the Stanford Neuromodulation Therapy (SNT) protocol.

Significant inter-subject variability in dorsolateral prefrontal cortex coordinates was found, emphasizing the necessity of individualized targeting.

DPABI and FSL pre-processing produced significant differences in target coordinates, which exceeded 1 cm, with mean Euclidean distances ranging from 1.45 to 3.82 cm across cohorts, and individual maximum deviations reaching 6.14 cm.

FSL pipeline consistently produced higher negative correlation coefficients than DPABI.