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New research · Ophthalmology
Clinical & experimental ophthalmology · 14h
Animal / preclinicalClinical & experimental ophthalmology · 2025

Investigating Microinvasive Intra-Ocular Biopsy.

Jared Ching, Shohei Kitahata, Hinako Ichikawa, Kazuaki Kadonosono
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OphthalmologyAnimal / preclinical

Microneedle anterior chamber puncture caused zero aqueous leakage

Investigating Microinvasive Intra-Ocular Biopsy.

Jared Ching … Kazuaki Kadonosono
Clinical & experimental ophthalmology · 2025
Background

Current minimally invasive methods of intraocular biopsy are confined to small gauge (G) needles and subretinal cannulae that can be prone to wound leakage at the biopsy site.

Methods

Human uveal melanoma (UM 92-1) and retinoblastoma (Y79) cancer cell lines were aspirated using microneedles of different sizes with a vitrectomy set up, and cell viability was analysed.

Results

no fluid leaked from the eye after the tiny-needle puncture

0%
Microneedle
100%
30G needle
66%
34G needle
More results

We find that microneedles can aspirate ocular cancer cells, both retinoblastoma and uveal melanoma, in vitro and retain a high level of cell viability, 72.83% (49G) compared to 97.00% (25G vitrector) in UM 92-1.

“
Conclusion

A microinvasive approach to biopsy intraocular specimens is feasible, warranting further in vivo studies.

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