Investigating Microinvasive Intra-Ocular Biopsy.

Animal / preclinicalClinical & experimental ophthalmology· Nov 2025

Investigating Microinvasive Intra-Ocular Biopsy.

Jared Ching, Shohei Kitahata, Hinako Ichikawa, Kazuaki Kadonosono

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Ocular OncologyAnimal / preclinicalQ1IF 3.1

Microneedle anterior chamber puncture caused zero aqueous leakage

Investigating Microinvasive Intra-Ocular Biopsy.

Jared Ching … Kazuaki Kadonosono

Clinical & experimental ophthalmology · Nov 2025 · Q1 in Ophthalmology

Background

Current minimally invasive methods of intraocular biopsy are confined to small gauge (G) needles and subretinal cannulae that can be prone to wound leakage at the biopsy site.

Methods

Human uveal melanoma (UM 92-1) and retinoblastoma (Y79) cancer cell lines were aspirated using microneedles of different sizes with a vitrectomy set up, and cell viability was analysed.

Results

no fluid leaked from the eye after the tiny-needle puncture

0%

Microneedle

100%

30G needle

66%

34G needle

More results

We find that microneedles can aspirate ocular cancer cells, both retinoblastoma and uveal melanoma, in vitro and retain a high level of cell viability, 72.83% (49G) compared to 97.00% (25G vitrector) in UM 92-1.

“

Conclusion

A microinvasive approach to biopsy intraocular specimens is feasible, warranting further in vivo studies.

Investigating Microinvasive Intra-Ocular Biopsy.

Investigating Microinvasive Intra-Ocular Biopsy.

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