ScholarDeck

Cohort studyClinical chemistry and laboratory medicine · 2026

Flow cytometry, cytology and histology in the diagnosis of ocular hematologic neoplasms: a 15-year monocentric experience with a focus on ocular lymphomas.

Alessandra Falda, Veronica Davanzo, Dario Marino … Daniela Basso

OphthalmologyCohort study

Flow cytometry phenotype matched ocular lymphoma histology in most cases

Flow cytometry, cytology and histology in the diagnosis of ocular hematologic neoplasms: a 15-year monocentric experience with a focus on ocular lymphomas.

Alessandra Falda … Daniela Basso

Clinical chemistry and laboratory medicine · 2026

Purpose

This study evaluated the effectiveness of flow cytometry in diagnosing ocular diseases, especially hematologic malignancies, and compared its performance with the other techniques.

Methods

In this monocentric study we collected patient characteristics from 165 patients referred to Padua University Hospital.

n = 165 patients

95.8%

Results

95.8%

flow cytometry cell markers matched the biopsy diagnosis in nearly all cases

n = 165 patients

More results

Hematologic neoplasms comprised 74 cases with ocular adnexal hematologic lesions and 12 cases with intraocular lymphomas and leukemias.

Histology showed 100 % PPV for both hematologic and non-hematologic neoplasms; flow cytometry reached 97 and 66 %, and cytology 80 and 91 %, respectively.

More results

Concordance between flow cytometry and cytology was lower (κ<0.61) than with histology (κ=0.677).

The lymphocytic infiltrate by flow cytometry was 39.5 % in non-neoplastic, 64 % in non-hematologic and 89.5 % in hematologic diseases with B lymphocyte prevalence of 31.3 %, 21.0 % and 93.3 %, respectively.

“

Conclusion · 1 of 2

Flow cytometry provides rapid results, studies numerous markers simultaneously, and analyzes ocular aspirates from minimally invasive samples.

Conclusion · 2 of 2

For this reason, it is desirable to permanently integrate this technique into the diagnostic process of ocular disorders, particularly when hematologic malignancies are suspected, as part of an integrated multidisciplinary pathway.

Post

Flow cytometry, cytology and histology in the diagnosis of ocular hematologic neoplasms: a 15-year monocentric experience with a focus on ocular lymphomas.

Flow cytometry phenotype matched ocular lymphoma histology in most cases

Related papers