Flow cytometry, cytology and histology in the diagnosis of ocular hematologic neoplasms: a 15-year monocentric experience with a focus on ocular lymphomas.

Hematologic neoplasms comprised 74 cases with ocular adnexal hematologic lesions and 12 cases with intraocular lymphomas and leukemias.

Histology showed 100 % PPV for both hematologic and non-hematologic neoplasms; flow cytometry reached 97 and 66 %, and cytology 80 and 91 %, respectively.

Concordance between flow cytometry and cytology was lower (κ<0.61) than with histology (κ=0.677).

The lymphocytic infiltrate by flow cytometry was 39.5 % in non-neoplastic, 64 % in non-hematologic and 89.5 % in hematologic diseases with B lymphocyte prevalence of 31.3 %, 21.0 % and 93.3 %, respectively.