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New research · Ophthalmology
Frontiers in oncology · 16h
AI / informaticsFrontiers in oncology · 2025

Oxidative stress-related genes in uveal melanoma: the role of CALM1 in modulating oxidative stress and apoptosis and its prognostic significance.

Yue Wu, Xiaoyan Cai, Menghan Hu … Yong Wang
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OphthalmologyAI / informatics

Oxidative stress gene risk signature predicts 5-year survival in uveal melanoma

Oxidative stress-related genes in uveal melanoma: the role of CALM1 in modulating oxidative stress and apoptosis and its prognostic significance.

Yue Wu … Yong Wang
Frontiers in oncology · 2025
Background

Uveal melanoma (uveal melanoma) is a rare yet aggressive form of ocular cancer with a poor prognosis.

Purpose

This study aims to investigate the role of oxidative stress-related genes (OSGs) in uveal melanoma, focusing on their involvement in key signaling pathways and immune infiltration and their potential as prognostic biomarkers and therapeutic targets.

Methods

A risk signature model was developed using data from The Cancer Genome Atlas (TCGA) cohort and validated using the International Cancer Genome Consortium (International Cancer Genome Consortium), GSE84976 dataset.

n = 175 samples
AUC = 0.844
Results
AUC = 0.844
model correctly separated higher- from lower-risk patients most of the time
n = 175 samples
More results

Prognostic hub OSGs, including ACACA, CALM1, and DNM2, were associated with poor survival outcomes in the training set and multiple validation data.

Revalidation using clinically collected samples confirmed that CALM1 exhibits superior diagnostic value.

More results

Immune infiltration analysis revealed increased CD4 + memory-activated T cells and mast resting cells in the high-risk group.

Additionally, CALM1 overexpression attenuated H 2 O 2 -induced oxidative stress and apoptosis in uveal melanoma cells.

“
Conclusion · 1 of 2

This study underscores the critical role of OSGs in the progression of uveal melanoma and their potential as prognostic biomarkers and therapeutic targets.

Conclusion · 2 of 2

The identified risk signature model and the protective role of CALM1 offer valuable insights for developing targeted therapies and enhancing patient clinical outcomes in uveal melanoma.

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