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Paper

Development and evaluation of a multi-panel real-time PCR assay for rapid detection of six bacterial species causing endophthalmitis.

New research · Ophthalmology

Experimental eye research · 1mo

StudyExperimental eye research· Sep 2026

Development and evaluation of a multi-panel real-time PCR assay for rapid detection of six bacterial species causing endophthalmitis.

Mohaddese Hajinezhadian, Samira Ghaedmohammadi, M Hossein Nowroozzadeh … Fatemeh Sanie-Jahromi

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UveitisStudyDiagnosisQ1IF 3.3

New PCR panel identifies endophthalmitis-causing bacteria in under 4 hours.

Development and evaluation of a multi-panel real-time PCR assay for rapid detection of six bacterial species causing endophthalmitis.

Mohaddese Hajinezhadian … Fatemeh Sanie-Jahromi

Experimental eye research · Sep 2026 · Q1 in Ophthalmology

Background

Endophthalmitis is a severe intraocular infection with the potential for rapid progression and irreversible blindness.

Purpose

This study reports the development and preliminary evaluation of a multi-panel real-time PCR assay designed to detect six clinically relevant bacterial species implicated in endophthalmitis (Pseudomonas aeruginosa, Streptococcus pyogenes, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus epidermidis, and Streptococcus sanguinis).

less than 4 h

Results

less than 4 h

results identifying the bacteria came back quickly, not days later

More results

Early and accurate pathogen identification is critical to guide therapy and improve prognosis.

“

Conclusion

Although the clinical sample size was small and the pathogen panel limited, these proof-of-concept data suggest that the proposed qPCR panel may complement conventional culture for more rapid pathogen detection in endophthalmitis.

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