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New research · Ophthalmology
Biomedicines · 3d
Cohort studyBiomedicines · 2026

Corneal Nerve Fiber Morphology and Biological Age in Healthy Adults.

Anait S Khalatyan, Yusef Yusef, Zoia V Surnina … Irina D Strazhesko
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OphthalmologyCohort study

Higher biological age is associated with lower corneal nerve fiber density.

Corneal Nerve Fiber Morphology and Biological Age in Healthy Adults.

Anait S Khalatyan … Irina D Strazhesko
Biomedicines · 2026
Background

Corneal confocal microscopy (confocal microscopy) quantifies subbasal corneal nerve fibers noninvasively and may inform peripheral neuroaging.

Purpose

We aimed to assess corneal nerve morphology in clinically healthy adults and determine whether confocal microscopy-derived parameters are associated with biological age (PhenoAge) beyond chronological age.

Methods

Eighty-four healthy volunteers (22-89 years) underwent confocal microscopy.

Results

each extra year of biological aging means fewer corneal nerve fibers detected on eye scan

50.8years
Chronological age
47.1years
PhenoAge
More results

Mean chronological age was 50.8 ± 15.5 years, and mean PhenoAge was 47.1 ± 16.3 years.

No systematic inter-eye differences were detected (all p > 0.05).

Across analyses, older age and higher PhenoAge were associated with lower main corneal nerve fiber measures, most consistently for main-fiber density.

More results

Participant-level sensitivity analysis (mean of both eyes) confirmed inverse associations of both chronological age and PhenoAge with main-fiber length and density (all p ≤ 0.035).

“
Conclusion · 1 of 2

Confocal microscopy-derived corneal nerve morphology demonstrates aging-related patterns in clinically healthy adults.

Conclusion · 2 of 2

The association between PhenoAge and main-fiber density may suggest a systemic biological aging component and warrants longitudinal validation.

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