Expanded methods for geometric location of 2D bone histology co-registration in 3D radiology volumes.

Replicated evaluation of an established method (M1) is also performed with new data.

M2 suits concave bisected sections by assuming a different orientation during tissue bandsaw than M1.

Three computed distances across 38 canine specimen dissections from each method were compared with three corresponding physical measurements collected during laboratory processing.

Mean errors for each method were of similar magnitude to 3D registration error reported in histology co-registration frameworks for other tissues.