Chemogenetic model of acute temporal lobe-like seizures in the area tempestas.

Surgical outcome studies in humans likewise suggest that this region contributes to seizure generation.

Twenty-two adult Wistar rats (Rattus norvegicus; 5 males, 17 females; approximately 4 months old, 215-490 g body weight) were used for this purpose.

Synchronized spike bursts were correlated with stage 4 and generalized tonic-clonic seizures.

GAD immunofluorescence revealed ≃87% of colocalization with hM4(Gi) receptors, consistent with seizures induced via reduction of inhibitory input in the area tempestas.