Multiplex mismatch amplification mutation polymerase chain reaction assay for the rapid detection of antimicrobial-resistant determinants in Cutibacterium acnes.

To prevent the progression of acne and the emergence of resistant strains, a rapid and easy method to assess antimicrobial resistance is needed.

Mutation-specific primers targeting 23S rRNA, 16S rRNA, and GyrA were designed based on the principles of the mismatch amplification mutation assay-polymerase chain reaction (MAMA-polymerase chain reaction).

A multiplex polymerase chain reaction incorporating the acquired resistance genes, erm(X), erm(50), and tet(W), was developed to evaluate the sensitivity and specificity of the method.