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New research · Ophthalmology
Discover oncology · 16h
Cohort studyDiscover oncology · 2025

Identification of an efficient immunohistochemical panel for precise differential diagnosis of sebaceous carcinoma of eyelid.

Sitao Yu, Qingyuan Lin, Weimin Ren, Tianhai Ji
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OphthalmologyCohort study

Combined TRPS1/GATA3/adipophilin marker panel accurately distinguishes sebaceous carcinoma from eyelid look-alikes.

Identification of an efficient immunohistochemical panel for precise differential diagnosis of sebaceous carcinoma of eyelid.

Sitao Yu … Tianhai Ji
Discover oncology · 2025
Background

Sebaceous carcinoma (sebaceous carcinoma) is a prevalent neoplasm of the eyelid, ranking second to basal cell carcinoma (basal cell carcinoma) and squamous cell carcinoma (squamous cell carcinoma) in incidence.

Methods

In this study, we performed a double-blind, retrospective analysis using tissue microarrays to evaluate the diagnostic accuracy of TRPS1, GATA3, and adipophilin in a cohort with 98 SCs, 56 BCCs, and 55 SCCs.

n = 98 SCs, 56 BCCs, and 55 SCCs
Results

correctly told sebaceous cancer from look-alikes nearly every time in this test

AUC-ROC 0.96 (95% CI 0.93 to 0.99)
null 0
0.93
0.99
CI excludes the null - significant
More results

In some Asian regions, the incidence of sebaceous carcinoma is as high as 50% with a high risk of invasion and recurrence.

More results

Binary logistic regression was applied to construct a cancer classification model, with model validity confirmed through likelihood ratio tests (Δ-2LL = 255.937, p < 0.001) and pseudo-R² metrics (Cox & Snell R² = 0.706, Nagelkerke R² = 0.803, McFadden R² = 0.578).

In poorly differentiated SCs, TRPS1 exhibited a markedly higher diagnostic efficacy than GATA3 and adipophilin.

“
Conclusion

Collectively, our findings indicate that the TRPS1/GATA3/adipophilin-based model demonstrated excellent discriminatory efficacy for eyelid malignancies (Kappa = 0.784, micro-average F1 = 0.882, AUC-ROC = 0.964 [95%CI:0.934-0.994]), achieving clinical-grade performance.

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