System biology analysis reveals that Grm6 is associated with glutamate accumulation-induced scotopic vision impairment in diabetic mice.

In total, 151 proteins were significantly altered in the retina of diabetic mice.

Among these 151 candidates, 22 genes presented a significant correlation with blood glucose level (p<0.05), which were enriched in alanine, aspartate, and glutamate metabolism (p=0.003).

Moreover, the glutamate catabolism-related genes Slc1a2, Gad1, and Glud1 were significantly negatively correlated with blood glucose at the transcript and proteome levels, which led to glutamate accumulation under hyperglycemia.

Moreover, Grm6 expression was significantly decreased at both the mRNA and protein levels in the diabetic retina.