Post

New research · Ophthalmology
Cutaneous and ocular toxicology · 2d
Animal / preclinicalCutaneous and ocular toxicology · 2026

Long-term ocular safety and systemic exposure of doxycycline hyclate ophthalmic solution in juvenile rabbits: a 270-day preclinical GLP study.

Yi-Chau Chen, Shi-Ju Huang, Hsin-Ming Chen … Chi-Jr Liao
Read paper
OphthalmologyAnimal / preclinical

Topical doxycycline eye drops caused no toxicity in rabbits over 9 months of twice-daily use.

Long-term ocular safety and systemic exposure of doxycycline hyclate ophthalmic solution in juvenile rabbits: a 270-day preclinical GLP study.

Yi-Chau Chen … Chi-Jr Liao
Cutaneous and ocular toxicology · 2026
Purpose

This study evaluated the chronic ocular safety and systemic exposure of a doxycycline hyclate ophthalmic solution following repeated topical administration in a juvenile model.

Methods

A 9-month Good Laboratory Practice (Good Laboratory Practice) repeat-dose study was conducted in juvenile Dutch Belted rabbits.

0.25
Results
0.25
mg/eye
highest dose tested still showed no eye or body-wide toxicity
More results

No treatment-related mortality, adverse clinical findings, or ocular tissue alterations were observed.

Ophthalmic examinations and clinical pathology showed no test article-related toxicity.

Systemic exposure increased dose-dependently, with T max generally observed 1-12 hours post-dose on Days 1, 180, and 270.

More results

Exposure demonstrated low accumulation and was independent of sex.

“
Conclusion · 1 of 3

Twice-daily topical doxycycline hyclate ophthalmic solution was well tolerated in juvenile rabbits for 9 months.

Conclusion · 2 of 3

No systemic or ocular toxicity was identified, establishing the No-Observed-Adverse-Effect Level (No-Observed-Adverse-Effect Level) at 0.25 mg/eye (1 mg/day).

Conclusion · 3 of 3

These findings support the safety of long-term ocular exposure to doxycycline hyclate for chronic applications.

Read paper
0 comments

No comments yet. Be the first.

Related papers

LatestFoundational
Cross-sectional
44.1%
had too much homocysteine in the blood, a far larger share than in dry AMD or healthy eyes
Cohort Study
92.3%
most laser-treated eyes had a favorable eye-structure outcome
Cohort Study
56%
lost three or more lines on the eye-chart vision test
Cohort Study
50%
older patients were more likely to lose three or more lines of vision
Study
81%
trials where mask air leaks were detected blowing toward the eyes
Cohort Study
13.5%
Central retinal artery peak blood-flow speed was 13.5% lower in diabetic patients.
Study
379 +/- 156 microm
lower thickness in the central retina, checked with an eye scan
Case Report
9 of 10 eyes
eyes had better measured vision after treatment