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New research · Ophthalmology
Micromachines · 3d
StudyMicromachines · 2026

A Microwell Platform for Characterizing the Dynamic Response of Corneal Keratocytes to Biochemical and Biophysical Cues.

Tarik Z Shihabeddin, Nathaniel S Tjahjono, Divya Subramanian … David W Schmidtke
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OphthalmologyStudy

Aligned collagen fibrils cut corneal keratocyte spreading area about 2-fold.

A Microwell Platform for Characterizing the Dynamic Response of Corneal Keratocytes to Biochemical and Biophysical Cues.

Tarik Z Shihabeddin … David W Schmidtke
Micromachines · 2026
Background

The interaction of corneal keratocytes with biochemical (e.g., composition, growth factors) and biophysical (e.g., topography) cues present in the cornea regulates their morphology during normal homeostasis and wound healing.

Purpose

To investigate the roles of topography and protein composition on the dynamic features of cell spreading, cell protrusions, and cell motility, we cultured keratocytes on either hydrophobic-coated glass, aligned collagen fibrils, or monomeric collagen with or without a fibronectin coating.

Results
~2-fold reduction
aligned fibers cut how much cells spread out, guiding wound healing
More results

In this study, we developed a novel method of fabricating substrates with micropatterns of Type I aligned collagen fibrils in a 6-well format that allowed for time-lapse imaging of dynamic changes in keratocyte morphology.

“
Conclusion

This approach will allow for systematic screening of the response of keratocytes and other cell types (e.g., tenocytes, cardiomyocytes, cancer cells) that normally are exposed to aligned collagen topographies.

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