Integrated multi-omics profiling reveals the role of the cfa gene in mediating penicillin antibiotics resistance and stress adaptability in Shigella sonnei.

The susceptibility of Res and Δcfa strains to ampicillin was compared, and the molecular regulatory mechanisms of cfa gene on antibiotic resistance in Shigella were explored by screening the differentially expressed mRNAs, proteins, and metabolites of the two groups.

Compared to the Res strain, the Δcfa mutant exhibited a 16-fold increase in susceptibility to amoxicillin, a 16-fold increase in susceptibility to both penicillin and aztreonam, a 4-fold increase in susceptibility to cefotaxime, and a 2-fold increase in susceptibility to cefazolin, ceftazidime, ceftriaxone and tetracycline.