Blood-Based DNA Methylation Models Improve Short-term Cardiovascular Risk Stratification in Individuals With Type 2 Diabetes.

Differential DNAm analysis identified 23,839, 21,529, 21,500, 12,114, and 7,101 differentially methylated CpG sites across the five analytical frameworks.

Least absolute shrinkage and selection operator logistic regression was used to develop five DNAm-based models.

Stability selection retained 22-26 CpG predictors per model.

Several CpGs were independently associated with either increased or decreased risk of major adverse cardiovascular events, including 12 CpGs identified across all of the models.